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Wednesday, July 29, 2020 | History

2 edition of Transport of S-adenosylmethionine in Saccharomyces cerevisiae. found in the catalog.

Transport of S-adenosylmethionine in Saccharomyces cerevisiae.

James Timothy Murphy

Transport of S-adenosylmethionine in Saccharomyces cerevisiae.

by James Timothy Murphy

  • 64 Want to read
  • 40 Currently reading

Published .
Written in English

    Subjects:
  • Amino acids.,
  • Yeast.

  • The Physical Object
    Paginationvii, 28 l.
    Number of Pages28
    ID Numbers
    Open LibraryOL16721826M

    Two experiments were completed to compare the supplemental effects of yeast Saccharomyces cerevisiae and sub‐therapeutic antibiotics in high‐fibre and low‐protein diets for broiler chicks. In experiment I, yeast was added at , and gkg ‐1 while penicillin, tylosin or neoterramycin were added at mg kg ‐1 into different batches of a high‐fibre diet containing g kg. View protein in PROSITE PS, MFS, 1 hit PS, SUGAR_TRANSPORT_1, 1 hit PS, SUGAR_TRANSPORT_2 p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry.

    Despite much study of the role of S -adenosylmethionine (SAM) in the methylation of DNA, RNA, and proteins, and as a cofactor for a wide range of biosynthetic processes, little is known concerning the intracellular transport of this essential metabolite. Screening of the Arabidopsis (Arabidopsis thaliana) genome yielded two potential homologs of yeast (Saccharomyces cerevisiae) and human. Abstract. To isolate an S-adenosylmethionine (SAM)-accumulating yeast strain and to develop a more efficient method of producing SAM, we screened methionine-resistant strains using the yeast deletion library of budding yeast and isolated SAM content in 81 of the strains was higher than that in the parental strain BY We identified ADO1 encoding adenosine kinase as one.

    View protein in PROSITE PS MFS, 1 hit PS SUGAR_TRANSPORT_1, 1 hit PS SUGAR_TRANSPORT_2, 1 hit This section displays by default the canonical protein sequence and upon request all isoforms described in the entry.   Monitoring levels of key metabolites in living cells comprises a critical step in various investigations. The simplest approach to this goal is a fluorescent reporter gene using an endogenous promoter responsive to the metabolite. However, such a promoter is often not identified or even present in the species of interest. An alternative can be a synthetic gene circuit based on a heterologous.


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Transport of S-adenosylmethionine in Saccharomyces cerevisiae by James Timothy Murphy Download PDF EPUB FB2

Abstract. The properties of a specific system for the transport of S-adenosylmethionine in yeast are process was pH- temperature- and energy-dependent, and showed saturation K m for the system was × 10 −6 the S-adenosylmethionine moieties tested, only S-adenosylhomocysteine competitively inhibited the uptake of the adenosylsulfonium by:   Abstract.

The properties of a specific system for the transport of S-adenosylmethionine in yeast are process was pH- temperature- and energy-dependent, and showed saturation K m for the system was × 10 −6 the S-adenosylmethionine moieties tested, only S-adenosylhomocysteine competitively inhibited the uptake of the adenosylsulfonium.

The properties of a specific system for the transport of S -adenosylmethionine in yeast are described. The process was p H- temperature- and energy-dependent, and showed saturation kinetics.

The K m for the system was × 10−6m. Of the S -adenosylmethionine moieties tested, only S -adenosylhomocysteine competitively inhibited the uptake of the adenosylsulfonium compound.

Murphy JT, Spence KD. Transport of S-adenosylmethionine in Saccharomyces cerevisiae. J Bacteriol. Feb; (2)– [PMC free article] Nakamura KD, Schlenk F. Active transport of exogenous S-adenosylmethionine and related compounds into cells and vacuoles of Saccharomyces cerevisiae.

J Bacteriol. Oct; (1)–Cited by: 1. Specificity and genetics of S adenosylmethionine transport in Saccharomyces cerevisiae. TransportofS-Adenosylmethionine in Saccharomyces cerevisiae J. MURPHYAND K. SPENCE DepartmentofBacteriology andPublic Health, WashingtonState University, Pullman, Washington Received for publication 25 October Theproperties ofa specific systemfor the transport ofS-adenosylmethionine in yeast are described.

Specificity and genetics of S adenosylmethionine transport in Saccharomyces cerevisiae Article (PDF Available) in Journal of Bacteriology (2) September with 28 Reads. Saccharomyces cerevisiae SAM, a mutant yeast strain with high S-adenosylmethionine synthetase activity (approximately 60 U/mL), was screened by flow cytometer following the method described in our previous study.

The slant medium consisted of 10 g/L yeast extract, 20 g/L peptone, 20 g/L glucose and 20 g/L agar. High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source.

putrescine transport Source: SGD, S-adenosyl-L-methionine transport Source: SGD, spermidine transport Source Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD. Sutherland FCW, Lages F, Lucas C, Luyten K, Albertyn J, Hohmann S, Prior BA, Kilian SG () Characteristics of Fps1-dependent and-independent glycerol transport in Saccharomyces cerevisiae.

J Bacteriol – PubMed Google Scholar. Polyamine transport in Saccharomyces cerevisiae. Polyamine uptake is mainly catalyzed by DUR3 and SAM3. Excretion of polyamines is mainly catalyzed by TPO1 and TPO5, located on either the plasma membrane or post-Golgi secretory vesicles.

Putrescine uptake into vacuole is catalyzed by UGA4. Download: Download full-size image; Fig. A gene for ethionine resistance isolated from the yeast Saccharomyces cerevisiae DKD‐5D‐H conferred on the yeast cells resistance to seleno‐ L ‐methionine and capability to produce S‐adenosyl‐ L ‐methionine in the cells.

An enzymatic study of the L ‐methionine synthetic pathway of L ‐methionine proto‐ and auxotrophs and in dried yeast cells with or without the gene.

Nakamura KD, Schlenk F. Active transport of exogenous S-adenosylmethionine and related compounds into cells and vacuoles of Saccharomyces cerevisiae.

J Bacteriol. Oct; (1)– [PMC free article] Pall ML. Amino acid transport in Neurospora crassa. Properties of two amino acid transport systems. Biochim Biophys Acta. Abstract. Saccharomyces cerevisiae B (α, ade-2, ura-1) in potassium phosphate buffer with glucose under aerobic conditions took up (−)S-adenosyl-l-methionine from the medium in sufficient quantity to permit the demonstration of its accumulation in the vacuole by ultraviolet same result was obtained with (±)S-adenosyl-l-methionine, (±)S-adenosyl-d-methionine, and.

Transport of S-Adenosylmethionine in Saccharomyces cerevisiae. By J. Murphy and K. Spence. Abstract. The properties of a specific system for the transport of S-adenosylmethionine in yeast are described.

The process was pH- temperature- and energy-dependent, and showed saturation kinetics. The Km for the system was × 10−6m. Transport of S-adenosylmethionine in Saccharomyces cerevisiae. Murphy JT, Spence KD. The properties of a specific system for the transport of S-adenosylmethionine in yeast are described.

The process was pH- temperature- and energy-dependent, and showed saturation kinetics. The K(m) for the system was x 10(-6)m. The stirred-tank bioreactor can provide the requirements of DO and suitable conditions for high density pure culture (Li et al., ; León-Vaz et al., ).The influence of the batch fermentation on the SAM production of Saccharomyces cerevisiae can be seen in Fig.

e was depleted at 8 h of inoculation and generated a large amount of ethanol, which was subsequently used as the main. Murphy JT, Spence KD. Transport of S-adenosylmethionine in Saccharomyces cerevisiae.

J Bacteriol. Feb; (2)– [PMC free article] Nakamura KD, Schlenk F. Examination of isolated yeast cell vacuoles for active transport. J Bacteriol. Apr. In the yeast Saccharomyces cerevisiae, Gcn2 is the sole eIF2α kinase (Dever, ).

It is activated in response to a variety of conditions, including nutrient starvation (amino acids, purines, and glucose) and exposure to sodium chloride, rapamycin, and volatile anesthetics (Hinnebusch, ; Palmer et al.

S-Adenosyl-l-methionine (SAM) is a commercialized fine chemical used as a nutritional supplement and a prescription drug. Saccharomyces cerevisiae is used as an industrial production host owing to the SAM accumulation capability of the Japanese sake brewing strains (Shiozaki et al.

).SAM is synthesized from methionine and ATP by methionine adenosyltransferase in S. cerevisiae. Ubiquitination regulates a host of cellular processes and is well known for its role in progression through the cell division cycle. In budding yeast, cadmium and arsenic stress, the availability of sulfur containing amino acids, and the intracellular concentration of S-adenosylmethionine are linked to cell cycle regulation through the ubiquitin ligase SCFMet  Journals & Books; Help Download full Vol Issue 1, 1 AugustPages Effect of S-adenosylmethionine and cyclic adenosine 3′,5′-monophosphate on RNA synthesis during glucose-derepression in Saccharomyces cerevisiae.This contributed volume reviews the recent progress in our understanding of membrane transport in yeast including both Saccharomyces cerevisiae and non-conventional yeasts.

The articles provide a summary of the key transport processes and put these in a systems biology context of cellular regulation, signal reception and homeostasis.